Which statement is NOT a limitation of monoclonal antibodies from a hybridoma?

Monoclonal antibodies derived from hybridomas are designed to be highly specific to a single epitope on an antigen. While option B states that they can be adapted to many immunological binding assays, this capability is actually a strength rather than a limitation of monoclonal antibodies. Their adaptability and specificity enable a wide range of applications, including diagnostics and therapeutic interventions.

In contrast, the other statements accurately depict limitations tied to monoclonal antibodies. They indeed may react with multiple antigens, particularly in cases where the epitopes share structural similarities, which can complicate specificity. The comment about reactivity with only one antigenic determinant on a multivalent antigen emphasizes the fact that while monoclonal antibodies target a single epitope, they might not effectively recognize the full range of possible epitopes present on a heterogeneous antigen. Additionally, the tendency for monoclonal antibodies not to cross-link or form precipitates limits their utility in certain assays, such as those requiring the formation of immune complexes for visual detection.

Thus, option B stands out as it highlights a versatile quality of monoclonal antibodies, distinguishing it from the inherent limitations presented in the other statements.